Last Updated on Monday, 01 April 2013 15:24
The Cytometry, Screening and Imaging Core Facility (CSI) Core Facility is a comprehensive research cell-culturing and cytometry facility, located on UTEP main campus in the fourth floor of Bioscience Research Building, rooms 4.208 and 4.220 (1,756 sq ft). The Core Facility was established in 2007, as an integral part of the Border Biomedical Research Center (BBRC), supported by the RCMI, administered by the NATIONAL INSTITUTE ON MINORITY HEALTH AND HEALTH DISPARITIES (NIMHD) Grant no. 8G12MD007592, a component of the NIH, under the Department of Biological Sciences and within the College of Sciences. This Facility was conceived to provide investigators, students and other researchers with “State-of-the-Art” technology to conduct cell-based experiments with modern equipment. The Core provides 24/7 access to all BBRC members and to other members of the institution.
The Cell Culture and HTS facility is essential for studying cellular, immune and physiological responses to stress, infectious agents, and toxicants. The recent integration of HTS equipment has allowed BBRC investigators to screen a large number of compounds for their therapeutic potential. The principal function of this Core is to provide assistance and training related with cell culture techniques and equipment operation. The Core also provide customized project support for experimental design and data analysis. During the past five years, the Cell Culture and HTS Core Facility greatly increased its capacity in services, equipment and space.
The CSI Core Facility sections
The Cell Culture section contains six self-contained culture suits. This section has the following equipment: fourteen CO2 water jacket incubators, ten Class II Bio-safety cabinets, six water baths, six inverted microscopes, and two incubators with shaker included (for insect cells).
The following is a list of the additional equipment within this section of the core: two benchtop refrigerated centrifuges, one microcentrifuge, one -80ºC and one -20ºC freezers, one electric autoclave, electroporation apparatus, two vortex and an inverted microscope with digital camera. Moreover, the long-term cryo-preservation capabilities include four liquid nitrogen containers and two ultra low -140ºC freezers.
The CSI section offers sophisticated equipment which includes fluorescence activated cell sorting (FACS; analyzer and sorter), and CSI bioimager, automated pipette system and two confocal microscopes. This equipement facilitates cell analysis and characterization as well as provides compound/drug screening capabilities. The CSI section is localized adjacent to the Cell Culture section to facilitate cell characterization and analyses.