Flow Cytometry Analyzer
Last Updated on Thursday, 15 September 2011 13:20
Flow Cytometry Analyzer (Cytomic FC500; Beckman Coulter)
The Cytomic FC 500 System offers automated tube-based acquisition for cell-based
Assays. This system is equipped with the following two lasers: an Argon ion laser (488nm) and
a Red solid state HeNe laser (631nm). Photodiodes also detect Forward Scatter (FS) and Side Scatter (SS) signals.
The following range of emissions can be detected:
| 525 nm/40 | (FL1; FITC, fluorescein isothiocyanate; Alexa Fluor488; GFP, green fluorescence protein; CFSE, carboxyfluorescein diacetate; Calcein) |
| 575 nm/40 | (FL2; PE, phycoerythrin; PI, propidium iodide) |
| 620 nm/20 | (FL3; PE, phycoerythrin; TexasRed; PI, Propidium Iodide) 675 nm/40 (FL4; APC, allophycocyanin; PE-Cy5, conjugate that combines phycoerythrin and cyanine dye 5; Alexa Fluor 647) |
| 755 nm/40 | (FL5; PE-Cy7, phycoerythrin conjugated with cyanine dye 7; APC-Cy7, conjugate of allophycocyanin and cyanine dye 7; APC-Alexa Fluor750, allophycocyanin conjugated to Alexa flour750. |
Services provided:
Hands-on training on flow cytometer system usage
.Assistance on flow cytometry experimental design.
Additional services provided upon request.
